Ko was born in Chicago and grew up in both Taiwan and Atlanta. She is the daughter of legendary Taiwanese entertainer Frankie Kao. Ko has discussed the issue of limited roles for Asian actors in the United States. Her role on The Great Indoors was originally written for a blond woman, but Ko's agent was able to get her an audition. After her audition, the producers changed their original idea of Emma's appearance. Ko said, "I think that's true diversity. You're just like everybody else. You just look Asian... It's the norm and how we all live. We just haven't seen it in Hollywood yet."
Ko joined the cast of the television series Hawaii Five-0 part way into its eighth season. She also guest-starred in the HBO comedy series Ballers. She stars on the FXX series Dave alongside Dave Burd.
In 2021, she starred alongside Canadian singer Justin Bieber in the music video of his song "Hold On".

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