Sartain was born in Tulsa, Oklahoma, the son of a Tulsa fire chief. He attended Cascia Hall Preparatory School, is a 1963 graduate of Will Rogers High School in Tulsa and was a member of the Epsilon Mu chapter of the Kappa Sigma Fraternity at the University of Tulsa, from which he graduated with a Bachelor of Fine Arts degree. In 1968, Gailard moved to New York City, where he worked as an assistant to illustrator Paul Davis.
Sartain's entry into entertainment was launched in Tulsa. Working originally as a cameraman at a local television station, he gained notoriety through the creation of a late night off-the-wall comedy program entitled The Uncanny Film Festival and Camp Meeting. Dressed as a wizard, wearing a dark blue robe and pointed wizard's cap, Sartain hosted the program as "Dr. Mazeppa Pompazoidi". Other cast members included fellow Tulsa native Gary Busey and Jim Millaway. The program was broadcast on the Tulsa CBS affiliate KOTV and later the ABC affiliate KTUL. It featured B-movies, with skits written and performed by Sartain, Busey and company between the movie segments.
Discovered by a talent scout during his stint as Mazeppa, Sartain was hired in 1972 as a regular on the television program Hee Haw. Sartain remained as a regular cast member of the popular show for nearly 20 seasons. He also served as a regular on other series including Cher (1975–76) and Shields and Yarnell (1978). Sartain played C.D. Parker for one episode during the pilot season of Walker, Texas Ranger. He supplied the voice of a social worker in the pilot episode of the animated series King of the Hill. Sartain also portrayed an advisor to Louisiana Governor Earl Long (played by Paul Newman) in the movie Blaze.

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