O'Brian was born in Indianapolis, Indiana. At the age of 9, O'Brian earned her brown belt in Shorei Goju Ryu Karate. Throughout her youth, she participated in numerous sports programs and music programs as a percussionist.
O'Brian attended Indiana University Bloomington, majoring in psychology and Germanic studies. She was one of the first members of the university's short-form improv troupe Hoosonfirst and received law enforcement certification through the university's Cadet Officer Program. While at the university, Katy received her black belt in Hapkido through the United States Hapkido Federation.
After graduation, O'Brian was a police officer at the Carmel Police Department in Carmel, Indiana. While working for the department, O'Brian obtained her personal trainer certification and began competing in bodybuilding figure competitions. She also began her training at the Indy Actor's Academy which gave her the confidence and experience to move to Los Angeles to pursue a career in acting. O'Brian moved to Los Angeles in July 2016 after signing with RCM Talent.

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