He was known for playing Lee Chan, the "Number One Son" in the Charlie Chan films, the original Kato in the 1939–1941 Green Hornet film serials, Brak in the 1960s Space Ghost cartoons, Master Po in the television series Kung Fu, and Mr. Wing in the Gremlins films.
He was the first Chinese-American contract player signed by RKO, Universal Pictures and Metro-Goldwyn-Mayer and was one of the most prominent Asian actors of American cinema in the mid-20th century.
Luke was born in Guangzhou, China, to a father who owned an art shop, but he was raised in Seattle. He was part of the Luke family, a relative of Washington assistant attorney-general Wing Luke, for whom Seattle's Wing Luke Asian Museum was named. He had four siblings who all emigrated from China to California during the Great Depression. His younger brother Edwin Luke also became an actor in the Charlie Chan series.

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