Born and raised in Yakima, Washington, Mackenzie is a 2001 graduate of Eisenhower High School, where she was selected as a first-team All-Big-9 selection all four seasons. She was named the 2000 Girl Golfer of the Year for both the Washington Junior Golf Association and the Pacific Northwest Golf Association. By the time she graduated from high school, Mackenzie had posted five top-10 finishes in national tournaments, claiming one championship.
Mackenzie continued her successful amateur career at the University of Washington in Seattle, where she graduated in 2006 with a bachelor's degree in business. She recorded 22 top-10 finishes during her collegiate career, placing her first on the Huskies' all-time list. Among her numerous amateur awards and achievements, Mackenzie was T13 at the 2005 U.S. Women's Open, was named the 2005 Pacific Northwest Golf Association's Women's Player of the Year, and won both the match play and stroke play competitions of the 2005 Trans National Championship. Mackenzie was the 2006 Pac-10 and NCAA West Regional champion and finished her senior year ranked as the No. 1 amateur in the nation by Golfweek.
In the summer following graduation, Mackenzie lead the U.S. to 11½–6½ victory at the 2006 Curtis Cup at Bandon Dunes in southern Oregon, posting a 3-0-1 record with victories in both of her singles matches. A week later in northern Oregon, she was the medalist in the stroke play portion of the U.S. Women's Amateur at Pumpkin Ridge. Her last event as an amateur was at an LPGA Tour event, the Safeway Classic in Portland, where she tied for 45th place.

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