Rona-Lee Shimon was born in Ramat Gan, Israel, to a Jewish family. When she was 3 years old, she started taking ballet lessons. At age 12, she joined Israeli dance troupe Bat Dor (בת דור). After graduating high school, she attended the Royal Ballet Academy in Amsterdam on a full scholarship. She then returned to Israel to dance professionally. Her sister, Sivan Noam Shimon, is also an actress, who acted in 2015 Israeli drama film Blush. Her brother, Almog Shimon taught her how to shoot a gun for Fauda.
In 2005, she was a contestant on the Israeli version of So You Think You Can Dance, called Born to Dance (נולד לרקוד). In 2006, Rona-Lee joined the cast of Our Song (השיר שלנו), a musical Israeli soap opera, as Shiri Gold, a singer in a military band.
Rona-Lee went to the theater school for three years and performed in plays like Cabaret and Chicago, with her latest role as Anita in West Side Story.

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